The results as follows : 1 ) the primary culture of qinchuan - scalper skin cells could be derived by fragment of tissue dispersed and cold digested single cell , collegenase i ( 150iu ml - 1 " ) and trypsin ( 0 . 05 % ) being used at the same time is best to dissociate qinchuan - scalper skin tissue , which is appropriate to obtain qinchuan - scalper skin cells 組織塊法、分散單細胞及dispase冷消化法單細胞均能獲得好的秦川牛皮膚組織原代培養(yǎng)物。其中150iu ? ml ~ ( - 1 )膠原酶i與0 . 05胰蛋白酶( 1 : 1 )同時應用能更好的分離秦川牛皮膚組織,是獲得秦川牛皮膚細胞的適宜方法。